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. 2015 Jan 2;290(8):5127–5140. doi: 10.1074/jbc.M114.599266

FIGURE 6.

FIGURE 6.

qRT-PCR analysis of FCGR3A gene transcripts. FCGR3A gene is expressed in activated CD4+ T-cells. A, all six donors analyzed showed an increase in the FCGR3A gene transcript from anti-CD3+ICs+C5b-9 activation and 3 of 6 from anti-CD3+antiCD-28 activation on day nine. Data are normalized with anti-CD3 for each donor population. B, time course analysis for FCFGR3A transcripts in CD4+ T-cells show maximum expression on day 9 with anti-CD3+ICs+C5b-9 and on day 4 with anti-CD3+anti-CD28 activation. Data are representative of two independent experiments. C, nine donors analyzed for the relative quantities (RQ) of FCGR3A transcripts in CD4+CD45RA+ T-cells (at 0 time point). Donors 2, 6, 7, and 8 showed relatively small increase. FCGR3A normalized with endogenous control GAPDH. FCGR3A in all donors was normalized using the donor 1 transcripts.