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Sequencing of amplified FCGR3 cDNA fragment. RT-PCR for FCGR3 transcripts from activated Jurkat, naïve CD4+, and leukocytes show an amplification product size of 330 (A, left panel). NTC, no template-control. Re-amplified product with nested M13-FCGR3 primers generated a 367 bp DNA fragment, which was sequenced with M13 primers (A, right panel). B, dideoxy sequence obtained aligned to NM_001127596. C, sequenced fragment aligned to cDNA coding for exons 4 and 5.
