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. 2015 Jan 2;290(8):5127–5140. doi: 10.1074/jbc.M114.599266

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 9. — Expression for Ifng/Tbx21 gene in activated CD4⁺ T-cells. qRT-PCR analysis for ifng (A) and Tbx21 (B) gene transcripts in five donors with two treatments. All of the five donors analyzed demonstrated an increase in ifng transcripts from 1.8–6.8-fold in the anti-CD3+ICs+C5b-9 treated group and no change to 6.37-fold in the anti-CD3+anti-CD28-treated group. For Tbx21 transcripts demonstrated an increase from no change to 2.8-fold, and no change to 1.4-fold increase was observed in anti-CD3+ICs+C5b-9 and anti-CD3+anti-CD28, respectively. Donor 3 did not express Tbx21 from anti-CD3+anti-CD28 treatment.