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In cell-attached mode, the non-inactivating form of PIEZO1 (DhPIEZO1) is unaffected by pH. A is a current trace from the non-inactivating form of PIEZO1 at the indicated pipette pH values (membrane potential of −80 mV, suction of −30 mm Hg). Unlike the wild-type channel at low pH, there was only a slow change in peak current from pulse to pulse. The normalized peak current is plotted in B and quantified in C with the S.D.
