FIGURE 6.

Triplex DNA is a preferred substrate for ChlR1 helicase. A–C and E–G, helicase reactions (20 μl) were performed by incubating the indicated concentrations of ChlR1-WT (A–C) or FANCJ-WT (E–G) with 0.5 nm two-stranded antiparallel G4 (OX-1 G2′, A and E), four-stranded parallel G4 structure (TP-G4, B and F) and flush triplex DNA (C and G) under standard helicase assay conditions as described under “Experimental Procedures.” D and H, quantitative analyses of data for OX-1 G2′ (×), TP-G4 (plus signs), and flush triplex (open squares) experiments with ChlR1-WT and FANCJ-WT, respectively. The data represent the means of at least three independent experiments with S.D. indicated by error bars.