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Extent of 18O incorporation in 3b under different conditions
| m/z | Enzyme reaction in H216O | Enzyme reaction in H218O | Incubation of non-labeled 3b in H218O | Incubation of 18O-labeled 3b in H216O |
|---|---|---|---|---|
| % | % | % | % | |
| 251 (0 18O) | 100 | 24 | 30 | 45 |
| 253 (1 18O) | 0 | 45 | 70 | 52 |
| 255 (2 18O) | 0 | 31 | 0 | 3 |
