Figure 5.

Supervised analysis identify (a) ‘bioinformatic approach (two-step). Schematic representation of PTCL subtypes classification based on miRNA profiling analysis. The flow diagram is constituted by two steps indicated in the figure by three arrows'. (b) Differentially expressed miRNAs comparing PTCL/NOS vs AITL. miRNAs with differences in expression were selected by the comparative cycling threshold methods (ΔΔCT) and filtered for statistical significance (two-tailed Student's t-test P-value <0.05 and FC >2). Eight miRNAs emerging from stepwise discriminant analysis. (c) Differentially expressed miRNAs comparing PTCL/NOS vs ALK-negative anaplastic large-cell lymphoma (ALCL/ALK−). miRNAs with differences in expression were selected by the comparative cycling threshold methods (ΔΔCT) and filtered for statistical significance (two-tailed Student's t-test P-value <0.05 and FC >2). Five miRNAs emerging from stepwise discriminant analysis. For each panel in the matrix, rows represent miRNAs and columns represent samples. The colour scale illustrates the relative expression level of miRNAs across all samples: red represents an expression level above the mean and green represents expression lower than the mean. Classification of PTCL subtypes by linear discriminant analysis in an independent set of cases. Scatterplots shows the distribution of PTCL/NOS vs AITL (d) and PTCL/NOS vs ALCL/ALK− (e) according to their discriminant scores in minimum miRNA set. Each case is represented in different colour according to its molecular classification. Green circles represent PTCL/NOS cases, red rhombus represents AITL cases and blue hexagons represents ALCL/ALK− cases.