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. 2015 Feb 20;3:9. doi: 10.3389/fcell.2015.00009

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Copyright © 2015 Winkler, Oltmer, Richter, Bischof, Xu, Burster, Leithäuser and Knippschild.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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mRNA expression levels of CK1δ in diffuse large B cell lymphoma tissues compared to lymphocytes from healthy donors. Comparison of CK1δ mRNA expression levels between (A) tumors of lymphoid tissues from 12 patients (P1–P12), (B) the established lymphoma cell lines HDLM2 and KM-H2 as well as the EBV-transformed lymphoblastoid B cells LCL1, LCL2, and BSM, and lymphocytes and other blood cells of healthy donors by quantitative real-time PCR as described in Materials and Methods. Results clearly demonstrate decreased mRNA expression levels of CK1δ in lymphoid tissues, lymphoma cell lines and EBV-transformed lymphoblastoid B cells compared to lymphocytes from healthy donors. Experiments were performed in duplicate. Data are presented as mean values of relative gene expression compared to HPRT mRNA levels, error bars indicate standard deviation (SD).