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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Cell Metab. 2014 Jun 19;20(1):85–102. doi: 10.1016/j.cmet.2014.05.011

Fig. 4. LTKO mice exhibit steatosis and alterations in IGF-1 and GH.

Fig. 4

7 week-old male lox/lox and LTKO or lox/+ and LTKO HET mice were HFF for 12 and 20 weeks respectively. Livers were extracted from fasted mice and either (a, c) processed for histology, (b) lipid analyses, (c, d, g) immunoblotting, or (e, h) real time PCR. (f) Lipogenesis was assessed ex vivo in liver slices. (i) Hepatocytes from chow-fed C57BL/6 were transfected with GFP- or Ptpn2-specific siRNAs and after 48 h processed for real time PCR. (j) Lox/lox and LTKO mice were HFF for 12 weeks, fasted overnight and serum IGF-1 and GH levels determined. Results are means ± SEM.

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