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. 2015 Feb 15;29(4):379–393. doi: 10.1101/gad.254425.114

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© 2015 Liu et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 1. — Stimulation of GLP activity by nucleosomes that are premethylated at H3K9. (A) Substrate description. (B,C) Histone methyltransferase activity assay at nucleosomes flanked by premodified nucleosomes with the wild-type GLP (B) or GLP catalytic mutant (C). (D) Histone methyltransferase activity assay at nucleosomes flanked by nucleosomes premodified at H3K27 with GLP. (E) Histone methyltransferase activity assay at nucleosomes flanked by H3K9 premodified nucleosomes with GLP 3A. (One microgram of recombinant enzymes and 1 μg of recombinant oligonucleosomes were used in the assay.)