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. 2015 Feb 15;29(4):379–393. doi: 10.1101/gad.254425.114

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© 2015 Liu et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 4. — H3K9me2 establishment and transcription silencing of Oct4 during ESC differentiation were delayed in GLP 3A cells. (A,B) ChIP-qPCR results showing H3K9me2 occupancy at the indicated loci in ESCs (A) or RA-treated cells at the indicated time points (B). (C) RT–PCR results showing Oct4 expression levels during differentiation. (D) DNA methylation levels at the Oct4 promoter during differentiation. Error bars represent the standard error.