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. 2015 Feb 15;29(4):440–450. doi: 10.1101/gad.254904.114

Figure 3.

Figure 3.

Analysis of the TLX–Atrophin interaction. (A) Atrophin contact residues are conserved in dTLX, rfbTLX, and hTLX, which define an autorepressed pocket. (B) TLX contact residues are conserved in dAtrophin (NP_996025), rfbAtrophin (XP_008194914), Atrophin-1 (NP_031907), and Atrophin-2 (NP_001078961), which define an ALXXLXXY motif (Atro motif; highlighted in gray) in the Atro box sequence. (C) Mutations in the dTLX autorepressed pocket affected its interactions with Atrophin-1 and Atrophin-2 in mammalian two-hybrid assays. (D) Mutations in the hTLX autorepressed pocket compromised its interactions with Atrophin-1 and Atrophin-2 in mammalian two-hybrid assays. (E) Mutations in the Atro motif of Atrophin-1 reduced its interactions with dTLX and hTLX in mammalian two-hybrid assays. (F) Mutations in the Atro motif of Atrophin-2 reduced its interactions with dTLX and hTLX in mammalian two-hybrid assays. All error bars indicate SD (n = 3). All experiments were repeated at least twice.