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. 1973 May;70(5):1526–1530. doi: 10.1073/pnas.70.5.1526

Biosynthesis of Reticulocyte Membrane Proteins by Membrane-Free Polyribosomes

Harvey F Lodish 1
PMCID: PMC433535  PMID: 4514321

Abstract

Intact rabbit reticulocyte cells synthesize two predominant species of membrane polypeptides; at least 10 reticulocyte membrane polypeptide species are not produced by the cells. Cell-free extracts of reticulocytes, free of any membranes or membrane-bound polyribosomes, synthesize large amounts of these two membrane polypeptides; one of these polypeptides appears to be modified, probably by loss of 20-40 amino acids, after it is incorporated into the membrane. Deprivation of hemin results in inhibition of synthesis by lysates of membrane proteins, globin, and all other cytoplasmic proteins.

Keywords: rabbit, hemin, globin, gel electrophoresis

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Selected References

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