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. 2014 Apr 10;1:8. doi: 10.3389/fmed.2014.00008

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Copyright © 2014 Chaudhury.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Shank3 is present in soma of myenteric nitrergic neuron. Note that shank3 has speckled appearance in cell periphery (A). (B–D) shows staining for nNOS, vAChT and colocalization of the three proteins. In (E), higher magnification view shows the speckled appearance of shank, which is still seen as diffuse distribution across the cell body, but it is not clear whether the distribution is only restricted to the surface. The higher power image shows diffuse distribution of nNOS in the both the cell cytosol and membranous region, colocalized with the submembranous location of shank3. Note that shank3 localization in nitrergic nerve terminals has not been examined. Modified with permission from Raab et al. (79).