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. 2014 Jun 20;1:17. doi: 10.3389/fmed.2014.00017

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Copyright © 2014 Chaudhury, De Miranda-Neto, Pereira and Zanoni.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparable levels of ramifications of nitrergic axonal processes and varicosities on the muscularis of vehicle treated and diabetic jejunum. In (A), the 16-bit images were pseudocolored sepia to focus on expression of the nitrergic varicosities and ignoring appearance of background during visualization. (B) Shows repetitive iterations of thresholding performed with NIH ImageJ. Cumulative thresholding helped subtract background and obtain accurate estimates of pixel intensities of nitrergic processes. (C) Shows comparative levels of space occupancy of nitrergic varicosities in wild type and diabetes, suggesting that no axonal retraction has taken place in diabetic tissues. The variance in the distribution of the axonal processes may be semiquantitatively expressed by this parameter of space occupancy derived from graph theory. Scale bar, 25 μm.