Figure 4.

Efficiency and outcomes of CliniMACS CCS validation for CMVpp65-specific T-cell enrichment. The percentage of IFN-γ secreting CMVpp65-specific T cells was detected after four hours of ex vivo stimulation with CMVpp65_pp using the QCP-A/A^- panel. The IFN-γ-based CliniMACS CCS and MiniMACS CSA systems were used for the isolation of IFN-γ-secreting CMVpp65-specific T cells. (A) The numbers of IFN-γ⁺ cells [x10⁶] within the CD3, CD4 and CD8 T-cell populations were analysed in all collected CliniMACS CCS fractions (leukapheresis, original fraction (OF), T-cell fraction (TCF), negative fraction (NF), waste fraction (WF), 48 h, 54 h, and 72 h post-leukapheresis (Stabi48, Stabi54, Stabi72)) to determine the efficiency of the CliniMACS process. Data sets of the representative analysis of the leukapheresis, OF, TCF, NF, WF fractions are shown (B) Outcome of IFN-γ-based CliniMACS CCS and MiniMACS CSA processes regarding the percentage [%] of IFN-γ⁺ cells among the CD3, CD4 and CD8 T-cell populations in samples collected at different steps of the validation process (leukapheresis, OF, TCF, NF, WF, Stabi48, Stabi54, Stabi72). Data sets of the representative analysis of the leukapheresis and TCF are shown. The results of independent experiments (n = 3) are expressed as the mean frequency of IFN-γ⁺ T cells ± SD. Asterisks indicate statistically significant differences between samples before and after T-cell enrichment (*p < 0.05).