Table 3.

Cell-size distribution at different time points of the fed-batch cultivation. Single and budding cells were categorized with a size of < 15 μm, whereas cell clusters were classified to be > 15 μm

Cultivation	Size distribution after batch [%]		Size distribution after non-induced fed-batch [%]		Size distribution after induced fed-batch [%]
	<15 μm	>15 μm	< 15 μm	> 15 μm	< 15 μm	> 15 μm
DoE1	53.0	47.0	41.5	58.5	79.8	20.2
DoE2	56.8	43.2	64.8	35.2	79.9	20.1
DoE3	54.7	45.3	60.5	39.5	64.7	35.3
DoE4	38.3	61.7	57.1	42.9	80.6	19.4
DoE5	53.8	46.2	74.8	25.2	73.3	26.7
DoE6	55.7	44.3	70.9	29.1	81.0	19.0
DoE7	36.3	63.7	42.5	57.5	75.8	24.2
DoE8	40.8	59.2	47.0	53.0	71.0	29.0
DoE9	49.9	50.1	69.5	30.5	90.4	9.6
DoE10	48.2	51.8	69.4	30.6	89.1	10.9
average	48.8 ± 7.6	51.3 ± 7.6	59.8 ± 12.3	40.2 ± 12.3	78.6 ± 7.8	21.4 ± 7.8
wildtype OCH1 strain	87.5	12.5	87.3	12.7	86.5	13.5