Fig 4. Hypothalamic POMC-derived peptides in nPE mutant mice.

(A) Representative ACTH immunohistochemistry in coronal hypothalamic sections. 3V, third ventricle. Scale bar, 200 μm. (B) Hypothalamic α-MSH and (C) β-endorphin content measured by radioimmunoassay. All data are from adult male mice (age 8 wk). n = 6–18. All quantitative data are presented as mean + 1 S.E.M. Genotype means were compared by two-tailed t-tests. *** P < 0.001 compared to +/+.