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. 2015 Feb 11;11(2):e1004935. doi: 10.1371/journal.pgen.1004935

Fig 6. Physiological responses of male nPE2 mutant mice to altered nutrition.

Fig 6

(A) Pomc expression measured by qRT-PCR in hypothalamus and (B) body composition measured by NMR of mice fed ad libitum (AL), fed a calorie restricted diet causing 20% weight loss in 1 wk (CR), or subjected to CR regimen followed by 24 hr ad libitum food access (RF). n = 6 of each genotype/treatment combination. (C) Cumulative food intake at each indicated time point during the 24 hr refeeding period. (D) Body composition measured by NMR in mice fed low fat (LFD; 10% kcal fat) or high fat (HFD; 60% kcal fat) diets for 16 wk. n = 2 for LFD, 6–7 for HFD. (E) Ucp1 expression in brown adipose tissue measured by qRT-PCR of mice fed LFD or HFD for 48 hr. n = 6 per treatment/genotype combination. Data are presented as mean + 1 S.E.M. Group means were compared by two-tailed t-tests. * P < 0.05, *** P < 0.001.