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Ultra-thin sections (~90 nm) of pharate adults (5 d-old pupae) were incubated with anti-TcCPR4 antibody. Anti-TcCPR4 antibody was detected using goat anti-rabbit IgG conjugated to 10 nm gold particles. The pore canal with pore canal fibers (PCF) in their core (2) and apical plasma membrane protrusions (APMP) (3) were enlarged. Scale bar in panel 1 = 1 μm and in panels 2 and 3 = 200 nm.
