Figure 1. clk-1 worms have increased antioxidant defenses.
The expression of antioxidant genes was examined in day 1 adult worms by quantitative real-time RT-PCR. Compared to WT worms (white), clk-1 worms (blue) showed increased expression of four different types of antioxidant genes including: (A) superoxide dismutases (sod), (C) peroxiredoxins (prdx), (D) catalases (ctl) and (E) thioredoxins (trx). The expression of SOD-3 protein is also significantly increased in clk-1 worms as indicated by an increase in GFP intensity in clk-1 worms expressing a SOD-3:GFP transgene under the sod-3 gene promoter (B). Error bars indicate SEM. * p<0.05, ** p<0.01, *** p<0.001.