Fig 3. Charge-substituting mutations on ATR1 recognition surfaces variably alter allele-specific HR.

(A) Co-inoculation of original ATR1-Cala2 Nda-GOF component substitutions (top row) and charge-altering variants. Constructs were co-inoculated as in Fig. 2A and photographed at 48 hpi. Relative strength of HR is indicated with symbols, +++, strong HR, +, weak HR, -, no HR. (B) Western blot of ATR1 mutants, as in Fig. 2B.