Figure 4. Effects of single amino acid substitutions in the Pab1 RRM2 domain on its interaction with eIF4G1.

(A) The crystal structure of the human RRM2 (PDB_ID 1CVJ) is shown. RRM2 positions with at least one amino acid substitution resulting in an enrichment score lower than 50% of the wild-type are colored in purple if the position was discovered by the combined analysis or in blue if the position was identified solely by the two-hybrid assay. All other RRM2 positions are shown in green. (B) Distribution of enrichment scores for amino acid substitutions that were not identified by the combined approach.