Figure 1. Downregulation of IMPα1 in clock neurons disrupts rest:activity rhythms and nuclear translocation of clock proteins, PER and TIM.

(A) IMPα1 knockdown leads to arrhythmia in constant darkness (DD). Genotypes are indicated on the top of each panel. The gray and black bars indicate subjective day and night, respectively. Representative activity records are shown. (B) Downregulation of IMPα1 impairs nuclear translocation of clock proteins, PER and TIM. Control flies and IMPα1 knockdown flies were collected at ZT0 and brains were dissected and stained with antibodies to PER or TIM (green) and PDF (red).