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. 2015 Feb 12;11(2):e1004974. doi: 10.1371/journal.pgen.1004974

Figure 2. An IMPα1 deletion mutant, Df(3L)α1S1, recapitulates the phenotypes of IMPα1 knockdown flies.

Figure 2

(A) Complete loss of IMPα1 results in weak rhythms in LD and complete loss of rhythms in DD. Flies were categorized as arrhythmic (AR), or having weak or robust rhythms based on FFT values as indicated beneath the panels. The white and black bars indicate day and night in LD cycles and the gray and black bars indicate subjective day and night in DD. (B) Activity records of individual UAS-importin α1-HA/+; Df(3L)α1S1 (left) and UAS-importin α1-HA/TUG; Df(3L)α1S1 (right) in DD. Expression of IMPα1 in all clock neurons using TUG in Df(3L)α1S1 flies rescues behavioral rhythms in DD. Rescue experiments were also conducted using pdf-Gal4 (Table 2). (C) Blocked nuclear entry of PER and TIM in LNvs of Df(3L)α1S1 flies. Brains were dissected from wild-type flies (iso31) and Df(3L)α1S1 flies and then stained for PER or TIM (green) and PDF (purple) on the 3rd day of LD at ZT0.