Table 1. Effects of downregulating importin α genes on free-running circadian locomotor rhythms.

Genotype	n	Rhythmic Flies (%) a	Period (hr) ± SEM	Power (FFT) ± SEM
α1 RNAi-1/+; dicer/+	16	100	23.36 ± 0.07	0.087 ± 0.012
α1 RNAi-2/dicer	16	100	23.06 ± 0.06	0.151 ± 0.009
α2 RNAi-1/+; dicer/+	16	100	23.22 ± 0.04	0.111 ± 0.011
α2 RNAi-2/dicer	16	100	23.46 ± 0.12	0.149 ± 0.014
α3 RNAi-1/dicer	16	100	23.80 ± 0.06	0.059 ± 0.008
α4 RNAi-1/+;dicer	16	100	23.62 ± 0.05	0.075 ± 0.009
Pdf-GAL4/+; dicer/+	12	100	23.77 ± 0.06	0.056 ± 0.010
Pdf-GAL4/α1 RNAi-1; dicer/+	19	84.2	23.98 ± 0.01	0.027 ± 0.003 b
Pdf-GAL4/+; dicer/α1 RNAi-2	14	100	23.68 ± 0.05	0.075 ± 0.006
Pdf-GAL4/α2 RNAi-1; dicer/+	15	93.3	23.86 ± 0.04	0.071 ± 0.010 c
Pdf-GAL4/+; dicer/α2 RNAi-2	15	100	24.66 ± 0.07	0.071 ± 0.010
Pdf-GAL4/+; dicer/α3 RNAi	16	100	24.23 ± 0.12	0.090 ± 0.008
Pdf-GAL4/α4 RNAi-1; dicer/+	15	100	24.04 ± 0.09	0.071 ± 0.007
tim-GAL4/+; dicer/+	15	100	23.85 ± 0.04	0.056 ± 0.008
tim-GAL4/α1 RNAi-1; dicer/+	14	0	-	-
tim-GAL4/+; dicer/α1 RNAi-2	16	100	23.72 ± 0.02	0.107 ± 0.010
tim-GAL4/α2 RNAi-1; dicer/+	15	100	24.31 ± 0.10	0.085 ± 0.008
tim-GAL4/+; dicer/α2 RNAi-2	16	100	25.17 ± 0.06	0.080 ± 0.009
tim-GAL4/+; dicer/α3 RNAi	lethal	-	-	-
TUG/+; dicer/+	15	100	23.68 ± 0.04	0.082 ± 0.011
TUG/α1 RNAi-1; dicer/+	15	0	-	-
TUG/+; dicer/α1 RNAi-2	16	100	23.43 ± 0.06	0.080 ± 0.008
TUG/α2 RNAi-1; dicer/+	15	86.7	24.43 ± 0.09	0.081 ± 0.010 c
TUG/+; dicer/α2 RNAi-2	16	100	24.92 ± 0.07	0.081 ± 0.009
TUG/+; dicer/α3 RNAi	14	71.4	23.96 ± 0.09	0.027 ± 0.007c,d
TUG/α4 RNAi-1; dicer/+	16	100	24.28 ± 0.13	0.139 ± 0.012

^a Flies with FFT value > 0.01 are counted as a rhythmic.

^b P < 0.005 compared to both α1 RNAi-1/+; dicer/+ and Pdf-GAL4/+; dicer/+ controls, by Student's t-test.

^c These flies showed arrhythmicity or weak rhythmicity 4–5 days after transferring to DD. Periods and FFTs during first 4–5 days in DD are shown.

^d P < 0.01 compared to both α3 RNAi-1/dicer and TUG/+; dicer/+ controls, by Student's t-test