Table 8.
List of primer pair for PCR amplification
| Primer | Sequence 5' to 3' | Targeted site | Product size (bp) | Reference |
|---|---|---|---|---|
| Esakf/ | GCTYTGCTGACGAGTGGCGGa | 16S rDNA | 929 | [33] |
| Esakr | ATCTCTGCAGGATTCTCTGG | |||
| EsgluAf/ | TGAAAGCAATCGACAAGAAGb | gluA | 1680 | [34] |
| EsgluAr | ACTCATTACCCCTCCTGATG | |||
| Saka 1a/ | ACAGGGAGCAGCTTGCTGCc | V1g | 952 | [14] |
| Saka 2b | TCCCGCATCTCTGCAGGA | V3h | ||
| ESSF/ | GGATTTAACCGTGAACTTTTCCd | ompA | 469 | [35] |
| ESSR | CGCCAGCGATGTTAGAAGA | |||
| Zpx F/ | GAAAGCGTATAAGCGCGATTCe | zpx | 94 | [27] |
| Zpx R | GTTCCAGAAGGCGTTCTGGT |
a&cRunning conditions; 94°C for 5 min; 35 cycles of 94°C for 1 min each; 60°C for 1 min; 72°C for 1.5 min; a final extension period of 5 min at 72°C.
bRunning conditions; 94°C for 10 min; 35 cycles of 94°C for 30 sec each; 60°C for 1 min; 72°C for1.5 min; final extension period of 5 min at 72°C.
dRunning conditions; 94°C for 5 min; 35 cycles of 94°C for 1 min each; 54°C for 45 sec; 72°C for1.5 min; final extension period of 5 min at 72°C.
eRunning conditions; 94°C for 15 min; 35 cycles of 94°C for 1 min each; 58°C for 1 min; 72°C for1.5 min; final extension period of 10 min at 72°C.