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Specific primers used to clone ARV and MS specific genes
| Primer name | Primer sequence | Amplicon length | Target gene |
|---|---|---|---|
| ARV C703F | 5’-TGTGGATCCATGGCGGGTCTCAAT-3’ | 981 bp | σC gene |
| ARV C703R | 5’-CCGGAATTCTAAGGTGTCGATGCC-3’ | ||
| MS vlhA F2 | 5’-CTGTTATAGCAATTTCATGTGGTG-3’ | 283 bp | phase-variable surface lipoprotein hemagglutinin (vlhA) |
| MS vlhA R2 | 5’-TGTTGTAGTTGCTTCAACTTGTCT-3’ |
