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Primers and probes used for the duplex real-time PCR assay
| Primer/probe name | Primer/probe sequence | Target gene | Amplicon length |
|---|---|---|---|
| ARV F | 5’-CGTTCCCTGTGGACGTATCA-3’ | σC | 69 bp |
| ARV R | 5’-GAGTACACCCCATACGCTTGGT-3’ | ||
| ARV P | 5’-(FAM) TCACCCGCGATTCTGCGACTCAT (Eclipse)-3’ | ||
| MS-F | 5’-ATAGCAATTTCATGTGGTGATCAA-3’ | vlhA | 143 bp |
| MS-R | 5’-TGGATTTGGGTTTTGAGGATTA-3’ | ||
| MS-P | 5’-(ROX) CAGCACCTGAACCAACACCTGGAA (Eclipse)-3’ |
