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. 2015 Jan 20;12:4. doi: 10.1186/s12950-015-0049-4

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© Kapewangolo et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Inhibition of NO release by O. labiatum extract and labdane diterpenoid. PBMCs were pre-incubated for 1 h with the indicated concentrations of the extract (OL) and compound (Lab) and then stimulated with 25 μg/mL PHA for 24 h. Untreated cells were used as a control. Nitrite accumulation was measured with the Griess reagent. Extract and compound concentrations used were non-toxic. The results were expressed as mean ± SD.