Figure 4.

Expression of renal gluconeogenesis restriction enzymes, phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase).

Notes: Light microscopic immunostaining (A) and Western blotting analysis for PEPCK (B) and G6Pase (C). The renal expression of PEPCK in the proximal tubules (stained in brown color, arrows) and distal tubules (asterisk) was increased in the DM rats compared with that observed in the control rats. ARB, telmisartan, treatment consequently suppressed the PEPCK expression. The renal expression of G6Pase was increased in the DM rats, whereas it was only faintly expressed in the control rats and diabetic rats treated with ARB. The figures for the PEPCK expression reflect data obtained under starvation conditions, while the results include some findings obtained under fed conditions (data not shown). The bar indicates 50 μm. *P<0.05, ^†P<0.01 versus control, ^‡P<0.05 versus DM. N=5–7 in each group.

Abbreviations: ARB, angiotensin receptor blocker; DM, diabetes mellitus.