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. Author manuscript; available in PMC: 2015 Feb 20.
Published in final edited form as: Cell Immunol. 2010 Dec 25;267(2):109–123. doi: 10.1016/j.cellimm.2010.12.004

Fig. 1.

Fig. 1

Schematic representation of monocyte maturation/differentiation into a macrophage in vivo and in vitro. Our culture system is represented in the top panel of the schematic. The in vivo system it was developed to mirror is represented in the bottom panel. Monocyte maturation/differentiation in the peripheral circulation in vivo is represented as a maturing monocyte, and in our culture system in vitro, this stage is represented by monocytes cultured non-adherently for 3 days. Upon adhesion to the BBB, monocyte maturation/differentiation in vivo is represented as an intermediate macrophage (MΦ) (transitional cell), and in our culture system in vitro, this stage is represented by monocytes cultured adherently for 3 days. In the CNS parenchyma, monocyte maturation/differentiation in vivo is represented as a macrophage (MΦ), and in our culture system in vitro, this stage is represented by monocytes cultured adherently for 6 days.