Skip to main content
. Author manuscript; available in PMC: 2015 Feb 20.
Published in final edited form as: Cell Immunol. 2010 Dec 25;267(2):109–123. doi: 10.1016/j.cellimm.2010.12.004

Fig. 3.

Fig. 3

Loss of cell surface CD14 after culture with MCSF for 3 days is not due to shedding and these monocytes maintain intracellular levels of CD14. (A) Supernatants from monocytes cultured for 3 days with MCSF were assayed for soluble CD14 (sCD14) by ELISA. The percentage of cells expressing cell surface CD14, as analyzed by FACS, and the levels of sCD14 (ng/ml) in the supernatants are shown. (B) Cell surface CD14 is analyzed by FACS in freshly isolated monocytes (Day 0) and monocytes cultured for 3 days non-adherently with MCSF (Day 3) and intracellular CD14 is analyzed in cells permeabilized before staining with anti-CD14-FITC.