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. Author manuscript; available in PMC: 2015 Feb 20.
Published in final edited form as: Biochim Biophys Acta. 2014 Mar 12;1840(7):2184–2191. doi: 10.1016/j.bbagen.2014.03.004

Fig. 5.

Fig. 5

Age- and CR-related changes of TFAM-binding to rat mtDNA regions (D-loop, Ori-L, and DR1) by mIP assay and following semi-quantitative PCR of mIP templates from the liver samples. Evaluation of TFAM-binding results in the three groups of animals after the mIP assay performed at three mtDNA regions. The groups of assayed animals included, respectively, six AL-18, six AL-28, and six CR-28 rats, and their values were represented in the scatter-plot format. The intensity of each DNA band, produced by the specific PCR and visualized on agarose gels (not shown), was analyzed by densitometry and used for detection of TFAM-binding by subtracting the value of the intensity of the aliquot precipitated without primary antibody from that of the TFAM-immunoprecipitated aliquot, both normalized to the value of the respective input aliquot made equal to 1.