Primers for PCR of mIP
|
|
|
|
|
7.1 For/7.3 Rev |
5′-CACTAATACTAACAACAAAACrAAC-3′ |
5′-GGGATTATGTAGGAGTCAAAGC-3′ |
(7133–7157) |
(7376–7355) |
8.0 For/8.2 Rev |
5′-CAACCGACTACACTCATTTCAAC-3′ |
5′-CTCATAGGGGGATGGCTATGC-3′ |
(8035–8057) |
(8246–8226) |
15.0 For/15.2 Rev |
5′-GAGTCGTAGCCCTAATCTTATC-3′ |
5′-GAATGAGGATAATTGAAAAGTAGC-3′ |
(15,004–15,025) |
(15,228–15,205) |
16.0 For/16.2 Rev |
5′-GCTCGAAAGACTATTTTATTCATG-3′ |
5′-GCTAAGATTTAAGTTAAAATTTTGTG-3′ |
(16,015–16,038) |
(16,226–16,201) |
5.0 For/5.3 Rev |
5′-GGATTCAAACCTACGAAAATTTAG-3′ |
5′-GTGGTTAGTTGAAAAGAGTCAAC-3′ |
(5092–5115) |
(5358–5336) |
Primers for RT-PCR
|
|
|
|
|
MtDNA For/mtDNA Rev |
5′-GGTTCTTACTTCAGGGCCATCA-3′ |
5′-TGATTAGACCCGTTACCATCGA-3′ |
(15,785–15,806) |
(15,868–15,847) |
Beta-actin For/beta-actin Rev |
5′-CCCAGCCATGTACGTAGCCA-3′ |
5′-CGTCTCCGGAGTCCATCAC-3′ |
(2181–2200) |
(2266–2248) |