| Component | Amount per reaction (μl) | Final concentration |
|---|---|---|
| Mixture prepared from Step 2 | 38 | |
| 5× nucleotide buffer (Prime-It Fluor kit) | 9.2 | |
| Cy3-dUTP (1 mM) | 0.8 | 16 μM |
| Klenow (Prime-It Fluor kit) | 2 |
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