| Component | Amount per reaction (μl) | Final |
|---|---|---|
| Template DNA | × | 2.5 μg |
| dATP (0.4 mM: Nick Translation kit) | 7.4 | 60 μM |
| dCTP (0.4 mM: Nick Translation kit) | 7.4 | 60 μM |
| dGTP (0.4 mM: Nick Translation kit) | 7.4 | 60 μM |
| labeled-dUTP (1 mM: see ▲ CRITICAL STEP below) | 3 | 60 μM |
| 10× Buffer (Nick Translation kit) | 5 | 1× |
| Enzyme mix (Nick Translation kit) | 5 | |
| Sterile water | added to 50 |
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