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. Author manuscript; available in PMC: 2015 Feb 20.
Published in final edited form as: Nat Protoc. 2011 Feb 10;6(3):270–284. doi: 10.1038/nprot.2010.195
Component Amount (μl) Final concentration
Formamide 500 50% (vol/vol)
50% (wt/vol) Dextran 200 10% (wt/vol)
20× SSC 100 2×
1% (wt/vol) BSA 100 0.1% (wt/vol)