Detection of nascent RNA, single-copy DNA and protein localization by immunoFISH in mouse germ cells and preimplantation embryos

. Author manuscript; available in PMC: 2015 Feb 20.

Published in final edited form as: Nat Protoc. 2011 Feb 10;6(3):270–284. doi: 10.1038/nprot.2010.195

Component	Amount per reaction (μl)	Final
Cot-1 probe (Box 1)	2	100 ng
Optional: gene-specific probe (Box 2)	2	100 ng
Ribonucleoside-vanadyl complex (200 mM)	2	20 mM
Hybridization buffer	Added to 20