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. Author manuscript; available in PMC: 2015 Jun 16.
Published in final edited form as: Nat Commun. 2014 Dec 16;5:5807. doi: 10.1038/ncomms6807

Figure 2. Direct reprogramming of MEFs to iMels.

Figure 2

a. RT-PCR analysis of melanocytic markers in MEFs which were reprogrammed by SMP3 combination. MEFs infected with SMP3 were collected for RT-PCR analysis at Day 5 after infection. MEFs and melan-a mouse melanocytes were used as negative and positive controls, respectively. The melanocytic markers included TYR, TYRP1, DCT, MITF (endo), SOX10 and PAX3. GAPDH here was used as an internal control. b. Morphologies of parental MEFs and SMP3-induced MEFs (SMP3-MEFs). MEFs (left panel) were infected with viruses containing SMP3, cultured for 19 days and then selected under G418 and photographed (right panel). Scale bar, 50 μm. c. DOPA activity in mouse iMels. Reprogrammed cells were stained by Dopamine and showed Dopa activity. Scale bar, 50 μm. d–f. Immunocytochemical staining of iMels derived from MEFs (MEF-iMels) using antibodies specific for TYR, S100 and Melan-A. Scale bar, 25 μm. g. qRT-PCR analysis of melanocytic markers, including MITF (endo), TYR, TYRP1, DCT, P, SOX10 (endo) and PAX3 (endo) in MEF-iMels and MEFs. Data shown are mean ± SD of the expression from three independent experiments.

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