Fig. 5. Large vesicle contains D-serine.

(A) Anti-D-serine antibody immunofluorescence (Green) of an astrocyte that had been patched with the 100 nM [Ca²⁺] Alexa Fluor-594 pipette solution. Alexa Fluor-594-stained large vesicles (Red, arrows) are Anti-D-serine-positive (Green, arrows). Data are representative chosen from 8 experiments. (B) A control slice treated with the second antibody only (2nd Antibody). Data are chosen from three experiments. (C) An astrocyte were patched with the 100 nM [Ca²⁺] Alexa Fluor-594 pipette solution added with HoAsp (400 μM) and DAAO (50 μg.ml). No high levels of anti-D-serine (Green) were found in large vesicles (Red, arrows). Data are representative chosen from 5 experiments. Scale bars in A, B, and C: 5 μm.