Table 1.

Primers Used for RT-PCR, Including Annealing Temperatures and Size of the Amplified Products.

Gene	Primer	Annealing Temperature (°C)	Product Size (bp)
Human COL1A1	Forward: TAA AGG GTC ACC GTG GCT	52	355
	Reverse: CGA ACC ACA TTG GCA TCA
Human COL2A1	Forward: AAG ATG GTC CCA AAG GTG CTC G	57	500
	Reverse: AGC TTC TCC TCT GTC TCC TTG C
Human COL3A1	Forward: GGA AAT GTA AAG AAG GCC CTG AAG CT	57	151
	Reverse: GTG TTC GAT ATT CAA AGA CTG TTT TGCT
Sheep COL1A1	Forward: AGG GAC CCA AAG GAG ACA CT	55	198
	Reverse: GCA CGG AAA TTC CTG TTG AT
Sheep COL2A1	Forward: CGT CAC CTA CCA CTG CAA GA	52	219
	Reverse: GGG AGA CGT GAG GTC TTC TG
Sheep COL3A1	Forward: CTT TTC GCT CTG CTT CAT CC	58	216
	Reverse: TTC TCC AAA CGG GAT TTC AG
GAPDH	Forward: CTC ACT GGC ATG GCC TTC CG	52	293
	Reverse: ACC ACC CTG TTG CTG TAG CC

COL1A1 = collagen type I; COL2A1 = collagen type II; COL3A1 = collagen type III; GAPDH = glyceraldehyde-3-phosphate dehydrogenase.