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. 2015 Jan 31;15(1):1. doi: 10.1186/s12896-015-0115-2

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© Lee et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Identification of putative alkaline phosphatase from metagenome. Sequencing of subclone (pSTV28-AP6) expressing alkaline phosphatase activity resulted in the assembly of a 5,428-bp contig. Three intact ORFs (ORF1 - ORF3) and two partial ORFs (PORF1 and PORF2) with conserved domains were identified through BLAST search.