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. 2015 Feb 14;16(1):82. doi: 10.1186/s12864-015-1274-1

Figure 2.

Figure 2

Negative correlation between the mean of the ΔC t values from the target genes and C t values of the reference genes after normalization via conventional subtraction. The lower right panel is based the Ct mean of all five reference genes while the others are based on individual reference gene. Ref, reference; dCt, ΔCt; r, Pearson correlation coefficient; p, p value from testing the correlation coefficient against 0.