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. 2015 Feb 14;16(1):82. doi: 10.1186/s12864-015-1274-1

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© Cui et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fold change and p value comparisons between ΔC _t and regression-based normalization methods. FC, fold change. For the ΔC_t method, the normalization factor is the mean C_t of the 5 reference genes; For the regression-based method, normalization factors are the multiple regressions of the mean C_t values of target genes (*) or the C_t values of each target gene (o) against the mean C_t of reference genes. The dashed line is the identity line. The vertical and horizontal lines in the right panel mark the significance level of nominal p value 0.05.