Fig. 2. Chemiluminescent responses of (a) 40 μM CHS-1, (b) 40 μM CHS-2, or (c) 40 μM CHS-3 to biologically relevant RSON species in 20 mM HEPES buffer (pH 7.4) containing 20% Emerald II Enhancer. Bars represent chemiluminescent emission at 545 nm and 30, 60, 120, 240, 360, 480 and 600 s after addition of RSON species. Data shown are for 5 mM glutathione, 1 mM cysteine and homocysteine, and 200 μM for other RSON species. Legend: (1) Na₂S; (2) Na₂S, glutathione, l-cysteine, and homocysteine; (3) glutathione; (4) S-nitrosoglutathione; (5) l-cysteine; (6) homocysteine; (7) HNO; (8) NO; (9) NaNO₂; (10) Na₂SO₃; (11) H₂O₂; (12) NaClO; (13) ^tBuOOH; (14) blank. Error bars are ± S.D.