Fig. 4. Detection of cellular H2S using a multi-well plate reader. (a) Luminescent responses of 40 μM CHS-3 and 0, 25, 50, 100, and 200 μM Na2S in 20 mM HEPES buffer (pH 7.4) containing 20% Emerald II Enhancer. (b) A549 cells were treated with a vehicle control (Cont), 200 μM Hcy (Hcy), or 200 μM Hcy after being pre-treated with 200 μM PAG (Hcy + PAG) for 20 min. 20 min after incubating with Hcy or vehicle, the cells were washed and treated with 40 μM CHS-3 and 125 μL Emerald II Enhancer. The reported values represent the average luminescent intensity of replicate experiments (n = 12, p = 0.044). Error bars represent ± S.E.M.
