Fig. 5. Imaging H2S using CHS-3. (a) Images 30 s after adding 40 μM CHS-3 to 0, 25, 50, 100, and 200 μM Na2S in 20 mM HEPES buffer (pH 7.4) containing 20% Emerald II Enhancer (n = 3). (b) Plot of total photon flux versus H2S concentration for the experiments described in (a). (c and d) Images of living C6 brown mice 30 s after administering i.p. injections of 0.08 μmol CHS-3 and (c) vehicle control or (d) 0.4 μmol Na2S in 100 μL 20 mM HEPES buffer (pH 7.4) containing 20% Emerald II Enhancer. (e) Quantification of the total photon flux from three replicates of the experiments described in (c) and (d). Statistical analyses were performed with a two-tailed Student's t-test (n = 3, p = 0.025). Error bars are ± S.D.
