FIG 4.

Inhibition of DENV-1 and DENV-2 replication. (A) Vero cells were preincubated with decreasing amounts of the compounds. DMSO served as a control. The cells were subsequently infected with DENV-2. Cell culture supernatants were collected and viruses titrated on Vero cells. All experiments were performed in triplicate assays. Error bars represent the standard deviation. Significances of differences in viral titers compared to the DMSO control were determined by the paired two-sample t test and are indicated by one (P < 0.05) or two (P < 0.01) asterisks above the bars. (B) Subtype specificity inhibition of replication by the PIs. Vero cells were preincubated with the compounds and infected with DENV-1 or DENV-2 (one genome copy [cp] per cell). Cell culture supernatants were collected, and viral genome amounts were determined by qRT-PCR. All experiments were performed in independent triplicate assays. Error bars represent the standard deviation.