FIG 4.

Impact of E138K and M184IV on phenotypic susceptibility to ETR, RPV, EFV, and NVP. The phenotypic susceptibility to ETR, RPV, EFV, and NVP of site-directed mutants containing amino acid substitutions M184I, M184V, E138K, and their combinations were determined. The lower cutoff values determined for this assay are indicated for each drug. Columns indicate mean values, while error bars indicate the standard errors of the mean (SEMs). Each mutant was tested in duplicate in at least two independent experiments. n.s., not significant; **, P < 0.01 (2-way analysis of variance); ***, P < 0.001 (2-way analysis of variance).